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Geographic History of COVID-19 Variants

COVID-19 has evolved continuously since late 2019 when it was first detected in Wuhan, China. The genomic sequencing results shown below reveal that the COVID-19 virus mutated slowly at first.  Beginning late 2020, however, variants of concern including Alpha, Beta, Gamma, and Delta rapidly appeared, all with spike protein mutations. Spike alterations may influence viral binding to human cells, a key step in cellular entry and resultant COVID infection. These spike changes may also affect how well vaccine antibodies bind spike protein to restrict cellular entry, thus potentially altering COVID protection from spike-targeting antibody vaccines.

The increased fitness of Alpha, Beta, Gamma, and Delta allowed them to spread rapidly in 2021. As illustrated above, there has been a viral competition of sorts resulting in a dominant variant, Delta. Beta and Gamma variants had regional spread at their sites of origin in South Africa and Brazil. Alpha and Delta variants spread globally in mid-2021. Today, Delta is the most common variant and appears largely responsible for increased COVID case-rates during the second half of 2021.

There is no reason to expect this evolutionary path, the rising of new variants, will stop anytime soon. Current vaccines are almost exclusively focused on the mutating surface spike protein, and a breakthrough infections do occur. Fortunately, antibody vaccine responses have so far largely maintained protection from hospitalization and death. As the virus continues to compete and evolve, new approaches targeting the non-mutating inner viral proteins, such as the nucleoprotein, should be pursued. Targeting these non-mutating proteins should not drive evolution of new viral variants and could provide a path forward for ending the COVID-19 pandemic.

PLEASANT HILL, CA / ACCESSWIRE / May 19, 2021 / Flow Pharma, Inc., a San Francisco Bay Area biotechnology company developing the FlowVax™ peptide vaccine platform technology, today announced the publication of an article describing research performed at the University of Texas Medical Branch at Galveston (UTMB). The article, entitled, “A synthetic peptide CTL vaccine targeting nucleocapsid confers protection from SARS-CoV-2 challenge in rhesus macaques,” was published May,18 2021 in the peer-reviewed journal Vaccines. Dr. Paul Harris and Dr. Trevor Brasel were equally contributing first authors on the paper.

“Several spike antibody evoking vaccines are being deployed in mass vaccination programs. Recent data suggest that as spike mutations increase, these vaccines may lose efficacy” said Paul Harris, Ph.D., Department of Medicine, Columbia University. “We are pleased with the positive results of this COVID-19 study, which were achieved by using a T-cell vaccine that does not target the mutating spike protein.

Trevor Brasel, Ph.D., M(ASCP)CM, assistant professor, Department of Microbiology & Immunology, UTMB said, “We are happy to be working with Flow Pharma on the pre-clinical testing phase of vaccines based on the FlowVax platform. Our team has extensive experience working with viruses in pre-clinical models and has been focusing on animal models for COVID-19 since the beginning of the pandemic. This study demonstrated that animals vaccinated with FlowVax COVID-19, and later challenged with SARS-CoV-2, were free of pneumonia-like infiltrates characteristic of COVID-19 infection, and presented with lower viral loads relative to controls.”

Dr. Brasel is author/co-author of over 80 peer-reviewed articles, editorials, book chapters, and presentations on topics including bioaerosol science and vaccine efficacy testing. Dr. Brasel’s ultimate goal is the development of high-profile FDA-compliant medical countermeasures specific to biodefense-related and emerging infectious diseases.

“We designed FlowVax COVID-19 to fight SARS-CoV-2 infections with killer T-cells rather than antibodies,” said Charles Herst, Ph.D., chief science officer, Flow Pharma. “This allows us to target the portions of the virus least likely to mutate, mitigating the risk of viral escape from vaccines that lead to a primarily antibody-driven response. In this study, we showed that a room-temperature-stable peptide vaccine targeting the SARS-CoV-2 nucleocapsid protein can provide protection against COVID-19 infection in nonhuman primates.”

“We are currently manufacturing clinical trial test doses of FLOVID-20, a version of FlowVax COVID-19 optimized for mass production,” stated Reid Rubsamen, M.D., M.S., CEO Flow Pharma, Inc. “The next step will be to conduct clinical trials of FLOVID-20 as a therapeutic for patients with acute and chronic COVID infections.”

About Flow Pharma

Flow Pharma, Inc. engineers and manufactures next generation T-cell stimulating biomedicines to treat and prevent viral diseases and cancers. Current products under development include FLOVID-20, to treat and prevent COVID-19, Ebola and Marburg virus vaccines and cancer vaccines to treat patients with breast cancer and cancers caused by human papilloma virus, including cervical cancer and throat cancer. Flow Pharma uses a proprietary mix of artificial intelligence, bioinformatics, and synthetic biochemistry to design and manufacture its expanding product portfolio.

About the University of Texas Medical Branch and the Galveston National Laboratory

The Galveston National Laboratory (GNL) is a sophisticated high containment research facility that serves as a critically important resource in the global fight against infectious diseases. The GNL is located on the campus of the University of Texas Medical Branch and operates under the umbrella of UTMB’s Institute for Human Infections and Immunity. The GNL is an anchor lab of the NIAID Biodefense Laboratory Network and is one of only two National Laboratories with Biosafety Level 4 (BSL-4) capabilities located on a U.S. university campus.

Forward Looking Statements

This press release may contain forward-looking statements, including information about management’s view of Flow Pharma, Inc. (“the Company”), future expectations, plans and prospects. In particular, when used in the preceding discussion, the words “believes,” “expects,” “intends,” “plans,” “anticipates,” or “may,” and similar conditional expressions are intended to identify forward-looking statements. Any statements made in this presentation other than those of historical fact, about an action, event or development, are forward-looking statements. These statements involve known and unknown risks, uncertainties and other factors, which may cause the results of the Company, its subsidiaries and concepts to be materially different than those expressed or implied in such statements. Unknown or unpredictable factors also could have material adverse effects on the Company’s future results. 

The forward-looking statements included in this presentation are made only as of the date hereof. The Company cannot guarantee future results, levels of activity, performance or achievements. Accordingly, you should not place undue reliance on these forward-looking statements. Finally, the Company undertakes no obligation to update these statements after the date of this release, except as required by law, and also takes no obligation to update or correct information prepared by third parties that are not paid for by Flow Pharma, Inc.

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Published on Accesswire

 

Flow Pharma’s latest research was recently features on news-medical.net:

With the coronavirus disease 2019 (COVID-19) pandemic showing no signs of abating, and some parts of the world displaying meteoric rises in the number of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infections, the need to achieve herd immunity is as urgent as ever. Though many spike-based vaccines have now been rolled out, their efficacy is threatened by the emergence of immune escape variants. Logistical constraints also hamper the production and use of the most effective vaccines.

A new study, released as a preprint on the bioRxiv* server, describes a synthetic peptide used as a platform to generate protective adaptive cellular immunity to the virus in rhesus macaques, which are the gold standard for preclinical testing in humans.

Study: A synthetic peptide CTL vaccine targeting nucleocapsid confers protection from SARS-CoV-2 challenge in rhesus macaques. Image Credit: iunewind / Shutterstock

Vaccine peptides

The scientists used synthetic vaccine peptide, within a biodegradable microsphere vehicle, along with adjuvants Toll-like receptor (TLR) 4 and 9 (to enhance immunogenicity). The peptide is based on antibody-binding sites that are known to induce an immune response.

The nucleoprotein (N) antigen was selected as the target of the T cells for the following reasons: SARS-CoV infection was associated with a memory T cell response sustained for two or more years after infection in survivors; the N protein sequence shows 90% homology between SARS-CoV and SARS-CoV-2, and for the selected epitopes only, 100% homology; there are much fewer mutations in this region that could lead to immune escape compared to the spike protein.

In fact, this region may be more essential to viral fitness, and therefore less susceptible to mutations.

The selected five epitopes recognize human leukocyte antigen (HLA) class I, and have been studied in either SARS-CoV or SARS-CoV-2. With the addition of a sixth peptide, these are expected to cover over 90% of HLA haplotypes globally.

Vaccine formulation

Microspheres of Poly-L-lactide-co-glycolide (PLG), including the synthetic peptides and the TLR9 agonist, were generated to deliver the peptides intratracheally by spraying or by lymph node (LN) injection (the latter in two macaques only, as a first dose).

The animals were then challenged with the virus both mucosally and intratracheally. The presence of infection was assessed by clinical features, by measuring the viral load, and by reverse transcriptase-polymerase chain reaction (RT PCR).  

Clinical features

Chest radiography

All vaccinated animals showed largely normal chest X-rays throughout the period of the study, while controls showed mild-to-moderate anomalies characteristic of COVID-19.

What are the implications?

The findings of this study indicated that SARS-CoV-2 infection in vaccinated animals tended to be milder, with lower viral loads, faster recovery from lymphopenia, and no pneumonia. In other words, the vaccination was protective against disease due to the virus.

This was underlined by the gene expression profiles, showing effective adjuvanting of the immunogenic peptides by the TLR4 and TLR9 agonists. They also found evidence of increased expression of both Mamu MHC Class I and Class II genes in the bronchoalveolar lavage (BAL) cells, the latter being observed for the first time in vaccinated animals.

The cytotoxic T lymphocyte (CTL) response did not show a bias towards T helper 2 (Th2) cell response. This implies a lower chance of antibody-dependent enhancement (ADE) of disease or vaccine-associated enhanced respiratory disease (VAERD),

The low mutation rate of this protein, as well as the ease with which the peptide sequences of these HLA Class I restricted epitopes for CTL epitopes can be customized, are attractive features of this vaccine candidate. Further, it is suitable for delivery by aerosolization rather than injection, and can be stored at room temperature without loss of stability, indicating the need for further validation and development.

 

Our FLOVID-20 team released a new research preprint showing that Rhesus Monkeys vaccinated with FLOVID-20 were free of pneumonia-like infiltrates characteristic of SARS-CoV-2 (COVID-19) infection and presented with lower viral loads relative to controls. It is submitted for peer review and publication.

Abstract

Background Persistent transmission of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has given rise to a COVID-19 pandemic. Several vaccines, evoking protective spike antibody responses, conceived in 2020, are being deployed in mass public health vaccination programs. Recent data suggests, however, that as sequence variation in the spike genome accumulates, some vaccines may lose efficacy.

Methods Using a macaque model of SARS-CoV-2 infection, we tested the efficacy of a peptide-based vaccine targeting MHC Class I epitopes on the SARS-CoV-2 nucleocapsid protein. We administered biodegradable microspheres with synthetic peptides and adjuvants to rhesus macaques. Unvaccinated control and vaccinated macaques were challenged with 1 x 108 TCID50 units of SARS-CoV-2, followed by assessment of clinical symptoms, viral load, chest radiographs, sampling of peripheral blood and bronchoalveolar lavage (BAL) fluid for downstream analysis.

Results Vaccinated animals were free of pneumonia-like infiltrates characteristic of SARS-CoV-2 infection and presented with lower viral loads relative to controls. Gene expression in cells collected from BAL samples of vaccinated macaques revealed a unique signature associated with enhanced development of adaptive immune responses relative to control macaques.

Conclusions We demonstrate that a room temperature stable peptide vaccine based on known immunogenic HLA Class I bound CTL epitopes from the nucleocapsid protein can provide protection against SARS-CoV-2 infection in non-human primates.

1. Introduction

Development of safe and effective vaccines for some viruses such as HIV and EBOV has been challenging [19]. Although vaccine development has been almost exclusively focused on eliciting a humoral immune response in the host through inoculation with whole protein antigen [51][69][59][29], CTL peptide vaccines producing a T-cell response may offer an important alternative approach [23]. For HIV and EBOV and influenza in particular, the potential of CTL vaccines has been discussed [21][7][56]. Although computational prediction alone has been used for T-cell vaccine design [2][14], we saw a unique opportunity to see if a preventative EBOV T-cell vaccine could be successfully designed based on the specific epitopes targeted by survivors of documented EBOV infection.

The notion of HLA restricted HIV control has been described [58]. Pereyra-Heckerman conducted an analysis of virus-specific CD8+ T-cell immunity in individuals living with HIV [43]. They reported that HIV controllers, individuals living with HIV not undergoing treatment who do not progress to AIDS, have CD8+ cells targeting different HLA restricted Class I epitopes on HIV compared with progressors, individuals with HIV who progress to AIDS in the absence of therapy. Pereyra-Heckerman suggested that this observation could guide the in silico development of a CTL vaccine for HIV and other diseases.

Acquired immunity has been documented after EBOV infection [4]. Antibody as well as T-cell responses have been described [44]. Sakebe et al. have shown that of 30 subjects surviving the 2013–2016 EBOV outbreak in West Africa, CD8+ T-cells from 26 of those survivors responded to at least one EBOV antigen, with 25 of the 26 responders targeting epitopes on EBOV NP [50]. One of the most commonly targeted EBOV eptitopes on EBOV NP in the survivor group (targeted by CD8+ cells from four survivors) was NP41-60 (IPVYQVNNLEEICQLIIQAF). They also suggested that a CTL vaccine could be designed using epitopes targeted by CD8+ T-cells identified in these EBOV controllers.

Human pathogen-derived peptide antigens that are also recognized by C57BL/6 T-cells have been previously described. These include peptides from vesicular stomatitis virus (VSV) RGYVYQGL [68], and human immunodeficiency virus (HIV) RGPGRAFVTI [5]. The existence of such epitopes makes a range of pre-clinical vaccine experiments possible without having to rely on non-human primates and expensive and complex-to-manage humanized mouse models. Wilson et al. showed that the EBOV nucleoprotein (NP) is an immunogen that provides protective, CTL-mediated immunity against EBOV in a C57BL/6 mouse model and that this protection was conferred by a peptide sequence within Ebola Zaire: NP43-53 (VYQVNNLEEIC) [73]. Wilson et al. came to this conclusion based on studying splenocytes harvested from mice vaccinated with Ebola Zaire NP using a Venezuelan equine encephalitis (VEE) vector. Their experiments showed that splenocytes from the vaccinated mice re-stimulated with NP43-53 had high levels of cytotoxic activity against target cells loaded with the EBOV NP peptide. Remarkably, NP43-53 also happens to be an 11 amino acid sub-sequence of the epitope identified by Sakebe et al. as most commonly favored for T-cell attack by survivors of the 2013–2016 EBOV outbreak in West Africa.

We set out to see if we could drive CTL expansion directed against NP43-53 to occur after vaccinating C57BL/6 mice with Ebola Zaire NP43-53 (VYQVNNLEEIC), and to subsequently conduct an in vivo EBOV challenge study to see if this peptide was protective.

We fabricated adjuvanted microspheres for this study as a room temperature stable dry powder using the Flow Focusing process to be 11μM in diameter so as to prevent more than one microsphere from being phagocytosed by any given antigen presenting cell (APC) at the same time [37]. By loading only one peptide sequence per microsphere, we maximized the peptide payload and mitigated the possibility of multiple, different peptide sequences being delivered to the APC simultaneously, which could possibly result in competitive inhibition at the motif which could interfere with antigen presentation and subsequent T-cell expansion (Supplementary Material Section 1).

We also set out to see if a similar approach to a CTL vaccine design for SARS-CoV-2 would be feasible based on an analysis of the HLA binding characteristics of peptide sequences on SARS-CoV-2 nucleocapsid.

2. Results

We used a previously described biodegradable dry powder, PLGA microsphere, synthetic vaccine platform adjuvanted with TLR-4 and TLR-9 agonists for this study [48]. In that article, we showed that the TLR-4 and TLR-9 agonists given together with a peptide in a mouse model did not produce T-cell expansion by ELISPOT and that microencapsulation of the peptide and the TLR-9 ligand, with the TLR-4 ligand in the injectate solution, was required to elicit an immune response to the delivered peptide antigen as determined by ELISPOT. That study also demonstrated that the microencapsulated peptides alone were insufficient to induce an adequate immune response without the presence of the TLR-4 and TLR-9 agonists administered as described. The TLR agonists used for this vaccine formulation are used in FDA approved vaccines and can be sourced as non-GMP or GMP material for pre-clinical and clinical studies.

We show here that the H2-Db restricted epitopes VSV (RGYVYQGL) and OVA (SIINFEKL), when administered to C57BL/6 mice, each produce a CD8+ ELISPOT response to the administered peptide antigen with no statistically significant CD4+ response measurable by ELISPOT as shown in Fig. 2c, and d.

We used this adjuvanted microsphere peptide vaccine platform to immunize C57BL/6 mice with NP43-53, the CTL Class I peptide antigen from the Ebola Ziare NP protein identified as protective by Wilson et al. [73]. Microspheres containing NP43-53 and CpG were prepared as a dry powder formulation and suspended before use in a PBS injectate solution containing MPLA, and administered intradermally via injection at the base of the tail into mice as described in a previous publication [48]. As illustrated in Fig. 1c, there was no statistically significant difference between the ELISPOT data for the vaccinated mice versus the response seen in the negative ELISPOT controls.

vaccine platform to immunize

Fig. 1. ELISPOT data from three groups of six mice each. Each of the three groups of mice were vaccinated (2 mg adjuvanted microspheres via ID tail injection) with a different 9mer peptide sub-sequence of NP43–53. ELISPOT data showed NP44–52 produced the best immune response (1a). Mice vaccinated (2 mg adjuvanted microspheres via ID tail injection) with the NP43–53 11mer produced the same immune response as ELISPOT plate negative control (1c). The same active formulation administered to mice for the challenge study (20 mg adjuvanted microspheres via intraperitoneal injection) produced a positive immune response compared with both adjuvanted microsphere and ELISPOT plate controls (1d). (n.s. = not significant).

Wilson reported that protection seen in her experiment was due to a peptide sequence within NP-43-53. We hypothesized that the NP43-53 epitope was inefficiently processed into MHC binding sub-sequences during antigen presentation. In order to explore possible H2-Db matches for peptide sequences contained within Ebola Zaire NP43-53 (VYQVNNLEEIC), we prepared three peptide vaccine formulations, each containing one of the three possible 9mer sub-sequences within NP43-53. These sequences are shown in Table 1. We then vaccinated, via intradermal (tail) injection, three groups of mice with microspheres containing one of the three 9mer sub-sequences of NP43-53 (6 per group). ELISPOT analysis was performed, stimulating harvested splenocytes with the three possible 9mer sub-sequences. Splenocytes from mice receiving the NP44-52 sub-sequence had a statistically higher ELISPOT response than mice vaccinated with the other two possible sub-sequence 9mers (P < 0.0001) as shown in Fig. 1a. This is consistent with the predicted H2-Db binding affinity of YQVNNLEEI as shown in Supplementary Material Table 3.

Table 1. Class I peptides used in the study. NP43-53 is the Class I 11mer described by Wilson et al. which we found not to produce an immune response in a C57BL/6 mouse model. NP43-51, NP 44-52 and NP 45-53 are the three possible 9mer sub-sequences of NP43-53.

splenocytes

We then loaded one population of adjuvanted microspheres with NP44-52 and a second population of adjuvanted microspheres loaded with VG19 from EBOV Zaire NP 273-291 (VKNEVNSFKAALSSLAKHG), a Class II epitope predicted to be relevant to NP43-53 based on the TEPITOPE algorithm using a technique described by Cunha-Neto et al. [14]. This peptide has a predicted favorable H2-Ib binding affinity as shown in Supplementary Material Table 5.

We showed that vaccination of 6 mice with the adjuvanted microsphere vaccine loaded with VG19 and NP44-52 showed an ELISPOT response to NP44-52 whereas 6 mice vaccinated with adjuvanted microspheres not loaded with peptide did not (Fig. 1d).

We also showed that mice vaccinated with VG19 alone did not show an ELISPOT response to NP44-52 (Fig. 2a) and, conversely, mice vaccinated with NP44-52 did not show a response to VG19 (Fig. 2b).

NP44-52

Fig. 2. Six mice treated with NP44-52 (2 mg adjuvanted microspheres via ID tail injection) were evaluated for their ELISPOT response to NP42-52 and VG19 (2a), and another group of six mice treated with VG19 (2 mg adjuvanted microspheres via ID tail injection) have their ELISPOT responses to NP42-52 and VG19 shown in b. In each of these groups, the mice generated an immune response only to the vaccinated peptide. A group of seven mice was evaluated for their immune response by cell type (using magnetic bead separation) for their ELISPOT responses evaluating total, CD8, and CD4 cell populations after vaccination (2 mg adjuvanted microspheres via ID tail injection) with OVA peptide (2c) and VSV peptide (2d). For both peptides, the immune response by ELISPOT was from the CD8+ cell population. (n.s. = not significant).

We conducted a pilot study demonstrating that intraperitoneal injection of the adjuvanted microsphere vaccine produced a statistically superior immune response by ELISPOT compared with the same dose delivered by intradermal tail or intramuscular injection in C57BL/6 mice (Supplementary Material Section 2). Based on the data from that study, and the fact that the volume of the intraperitoneal space would allow larger amounts of microsphere suspension to be delivered, we chose to proceed with intraperitoneal administration for the challenge portion of this study delivering 20 mg of microspheres per dose.

We dosed three groups of mice, ten mice per group, with the adjuvanted microsphere vaccine formulation containing NP44-52 and VG-19, with each peptide in a distinct microsphere population, and challenged these mice 14 days after vaccine administration with escalating IP administered doses of mouse adapted EBOV (maEBOV) (Group 3-100 PFU, Group 5-1000 PFU and Group 7-10,000 PFU). The composition of the vaccine used for the exposure study is described in Supplementary Material Section 3. A second set of three control groups of mice (groups 2, 4 and 6), ten mice per group (mock groups), received PBS buffer solution alone and served as control animals for the study and were similarly challenged with maEBOV. Group 1 animals served as study controls and received no PBS buffer, vaccine or maEBOV injections. All mice were sourced from Jackson Labs and were 6–8 weeks of age and 15–25 grams at the time of vaccination. The dosing regimen is outlined in Table 2.

Table 2. C7BL/6 maEBOV challenge study dosing regimen with PBS (buffer) controls. All challenges were done with Ebola virus M. musculus/COD/1976/Mayinga-CDC-808012 (maEBOV) delivered IP. Mice in Group 1 received no injections.

Peak mortality across all groups tested was seen in mice challenged with 1,000 PFU maEBOV versus PBS buffer control as shown in the survival curve in Fig. 3a. Clinical observation data shown in Fig. 3b and c and daily weight data shown in Fig. 3d and e show protection from morbidity in all active vaccinated mice exposed to 1,000 PFU maEBOV.

 
survival curve
 
Fig. 3. 1000 PFU post-challenge data (20 mg active adjuvanted microspheres via intraperitoneal injection versus PBS buffer solution) collected beginning 14 days after vaccination.
 
PBS buffer mock-vaccinated mice showed mortality increasing from the 100 PFU to 1,000 PFU as shown in Fig. 4a and Fig. 3a. We saw a paradoxical effect in control animals with survival increasing between 1,000PFU (Fig. 3a) and 10,000 PFU (Fig. 5a). We believe this was caused by innate immunity triggered by the very large maEBOV challenge. All mice in all vaccinated groups across both experiments survived and showed no morbidity by clinical observation scores and weight data.
 
buffer mock-vaccinated
 
Fig. 4. 100 PFU post-challenge data (20 mg active adjuvanted microspheres via intraperitoneal injection versus PBS buffer solution) collected beginning 14 days after vaccination.
 
pbs buffer
 

Fig. 5. 10,000 PFU post-challenge data (20 mg active adjuvanted microspheres via intraperitoneal injection versus PBS buffer solution) collected beginning 14 days after vaccination.

For each of the three challenge levels, the difference between the number of survivors in the vaccinated group versus the PBS control group was statistically significant by chi square (100 PFU P = 0.001; 1000 PFU P = 0.0003; 10,000 PFU P = 0.003).

We saw what appears to be an innate immune response at the 10,000 PFU EBOV exposure level. It has been suggested that EBOV can mediate an innate immunity response through stimulation of TLR-4 [33]. Because the adjuvanted microsphere vaccine used in this experiment incorporates a TLR-4 agonist, we dosed 10 mice with adjuvanted microspheres without peptides and found the level of protection after exposure to 100 PFU EBOV to be statistically no different from that seen in PBS buffer controls (Supplementary Material Fig. 1). We conclude that level of protection conferred by the adjuvanted vaccine described in this study is dependant on delivering peptides with the microspheres.

The data in Supplementary Material Fig. 1 also shows, in two separate experiments conducted months apart with the same 100 PFU maEBOV challenge dose and the same (active) vaccine formulation, that the vaccinated animals in both active groups had 100% survival and no morbidity by clinical observation. This provides some evidence that the protective effect of vaccination using this adjuvanted microsphere vaccine is reproducible.

Serum samples from sacrificed animals exposed to EBOV who did not receive vaccine were quantitatively assayed for various cytokines using BioPlex plates. Animals having unwitnessed demise did not have serum samples collected. A Pearson Correlation Analysis was performed to assess relationships between specific cytokine levels and survival. The results are shown in Table 3.

Table 3. Cytokines with statistically significant (positive or negative) correlation with survival in non-vaccinated mice are shown here along with (Pearson Correlation Analysis) p-values.

Cytokine/Survival Correlations for Control Groups
Cytokine p-Value Correlation
Mo IL-6 0.050 Decreased with survival
Mo MCP-1 0.019 Decreased with Survival
Mo IL-9 0.015 Increased with survival
Mo MIP-1b 0.009 Decreased with survival
Mo IL-12(p40) 0.006 Increased with Survival
Mo G-CSF 0.005 Decreased with Survival
Mo IL-1b 0.005 Increased with Survival
Mo IFN-g 0.003 Increased with Survival
Mo GM-CSF 0.002 Increased with Survival
Mo IL-12(p70) 0.001 Increased with Survival
Mo TNF-a 0.001 Increased with Survival
Mo IL-17 0.000 Increased with Survival
Mo IL-10 0.000 Decreased with Survival

We observed low levels of IL-6 in surviving mice. NHPs infected with EBOV have been determined by other researchers to have elevated levels of IL-6 in plasma and serum [27][17]. EBOV infected humans have also shown elevated IL-6 levels and these elevated levels have been associated with increased mortality [71].

Similarly, we observed low levels of MCP-1, IL-9 and GM-CSF in survivors. Increased serum and plasma levels of MCP-1 have been observed in EBOV infected NHPs [22][27][17] and elevated levels of MCP-1 were associated with fatalities in EBOV infected human subjects [71]. Human survivors of EBOV have been found to have very low levels of circulating cytokines IL-9 and elevated levels of GM-CSF have been associated with fatality in humans exposed to EBOV [71].

We saw increased levels of IFN-γ in survivors. Other vaccine studies have associated IFN-γ with protection [70][38].

We achieved protection against maEBOV challenge with a single injection of an adjuvanted microsphere peptide vaccine loaded with a Class I peptide in a region on EBOV nucleocapsid favored for CD8+ attack by survivors of the 2013–2016 West Africa EBOV outbreak. There is evidence that a CTL response could be beneficial in the context of a coronavirus infection. [13][41][64][11][28][36] Peng et al. have found survivors of the SARS-CoV-1 outbreak who had circulating T-cells targeting SARS-CoV-1 nucleocapsid two years after initial infection. [42] We decided to investigate the feasibility of designing a SARS-CoV-2 peptide vaccine targeting SARS-CoV-2 nucleocapsid.

All available SARS-CoV-2 protein sequences were obtained from the NCBI viral genomes resource within GenBank, an NIH genetic sequence database [8]. Retrieved sequences were processed using multiple sequence alignment (MSA) via Clustal for the nucleocapsid phosphoprotein [34]. The nucleocapsid phosphoprotein sequences were trimmed down to every possible peptide sequence 9 amino acids in length. 9mers were chosen because they typically represent the optimal length for binding to the vast majority of HLA [1][18][3]. The resulting peptides were compared to the MSA to ensure than these sequences are conserved within all of the sequencing samples available and not affected by an amino acid variant that could complicate subsequent analysis, specifically the calculation of population coverage. A selection of HLA were selected to encompass the vast majority of the worlds population at over 97% coverage.

Peptides were run through artificial intelligence algorithms, netMHC and netMHCpan which were developed using training data from in vitro binding studies. The pan variant of netMHC is able to integrate in vitro data from a variety of HLA to allow for predictions to be made if limited in vitro data is available for the specified target HLA [30][1]. This in silico analysis utilizes the neural networks ability to learn from the in vitro data and report back predicted values based on the imputed SARS-CoV-2 nucleocapsid phosphoprotein peptides. Peptides with a predicted HLA IC50 binding affinity of 500 nm or less in either of the algorithms, were included in the candidate list of targets for the vaccine [30][1][40].

A subset of these SARS-CoV-2 peptide sequences are present on SARS-CoV-1 nucleocapsid phosphoprotein and as a result had in vitro binding data in Immunology Epitope Database and Analysis Resource (IEDB) collected after a previous outbreak [65]. Predicted values of these peptides were cross referenced with actual in vitro binding measurements from identical 9mer peptides when that data was available.

3. Summary and discussion

Most preventative vaccines are designed to elicit a humoral immune response, typically via the administration of whole protein from a pathogen. Antibody vaccines typically do not produce a robust T-cell response. [72] A T-cell vaccine is meant to elicit a cellular immune response directing CD8+ cells to expand and attack cells presenting the HLA Class I restricted pathogen-derived peptide antigen. [47] Difficulty in obtaining a reliable immune response from peptide antigens and the HLA restricted nature of CTL vaccines have limited their utility to protect individuals from infectious disease [77]. However, observations derived from individuals able to control HIV infection [43] and EBOV infection [50] demonstrating that control may be associated with specific CTL targeting behavior, suggest that there may be an important role for HLA-restricted peptide vaccines for protection against infectious disease for which development of an effective traditional whole protein vaccine has proved to be difficult. The adjuvanted microsphere peptide vaccine platform described here incorporates unmodified peptides making possible rapid manufacture and deployment to respond to a new viral threat.

NP44-52 is located within the EBOV nucleocapsid protein considered essential for virus replication. This epitope resides in a sequence conserved across multiple EBOV strains as shown in Supplementary Material Fig. 6. A 7.3 Å structure for NP and VP24 is shown for context in Fig. 6[67]. A 1.8 Å resolution structure rendering for EBOV NP shown in Fig. 6b illustrates that NP44-52 is a buried structural loop, which is likely to be important to the structural integrity of the EBOV NP protein [16]. This structural role of NP44-52 likely explains its conservation across EBOV strains.

Nucleocapsid proteins

Fig. 6. The Class I epitope used for this study is located within NP. Nucleocapsid proteins NP and VP24 are shown together in (a). A detailed view of NP with the study epitope position highlighted in shown in (b).

CTL targeting of the EBOV NP protein has been described [42][64][28][49][24]. Nucleocapisid proteins are essential for EBOV replication [61]. Recent advances in T-cell based vaccines have focused on avoiding all variable viral epitopes and incorporating only conserved regions [7][25]. EBOV NP may be more conserved than nucleocapsid proteins VP35 and VP24 making it more suitable as a CTL vaccine target [9][73]. The nucleocapsid proteins in SARS-CoV-1 are also essential for that virus to function normally [10]. This suggests that a CTL vaccine targeting coronavirus nucleocapsid could be effective against SARS-CoV-1 or SARS-CoV-2.

We have shown that an H2-Db restricted Class I peptide exists within the NP41-60 epitope identified by Sakebe et al. as the most commonly favored NP epitope for CD8+ attack by survivors of the 2013–1016 EBOV outbreak in West Africa. We have demonstrated, when delivered in conjunction with a predicted-matched Class II epitope using an adjuvanted microsphere peptide vaccine platform, NP44-52 protection against mortality and morbidity for the maEBOV challenge doses tested in a C57BL/6 mouse model. We accomplished this with an adjuvanted, microsphere-based, synthetic CTL peptide vaccine platform producing a protective immune response 14 days after a single administration.

EBOV can cause severe pulmonary problems in exposed subjects [39]. These problems can be especially severe when the virus is delivered by aerosol [15][31]. Interaction of EBOV specific antibody, NHP lung tissue and EBOV delivered to NHPs via aerosol can produce a more lethal effect than in NHPs without circulating anti-EBOV antibody exposed to aerosolized EBOV (unpublished conference presentation). This suggests that a CTL vaccine may be more effective for prophylaxis against filovirus protection than an antibody vaccine if the anticipated route of EBOV exposure is via aerosol.

Sakebe et al. identified A∗30:01:01 as the only HLA type common to all four survivors in their study with CD8+ targeting of NP41-60. The A∗30 supertype is relatively common in West Africa: 13.3% for Mali, 15.4% for Kenya, 16.3% for Uganda, and 23.9% for Mozambique [32]. Although peptide vaccines are by their nature HLA restricted, it may be possible to create a CTL vaccine directed against EBOV for use alone or in conjunction with a whole protein vaccine to produce an antibody response in tandem, by incorporating additional Class I peptides from epitopes targeted by controllers to broaden the HLA coverage of the vaccine. MHC binding algorithms hosted by the IEDB predict that YQVNNLEEI will bind strongly to the MHC of HLA-A∗02:06, HLA-A∗02:03 and HLA-A∗02:01 individuals (Supplementary Material Table 2[65]. HLA-DR binding database analysis also suggests that VKNEVNSFKAALSSLAKHG demonstrates sufficiently promiscuous binding characteristics cover that same population (Supplementary Material Table 4[65]. Taken together, a peptide vaccine based on YQVNNLEEI and VKNEVNSFKAALSSLAKHG could produce a cellular immune response in about 50% of the population of the Sudan and about 30% of the population of North America.

The internal proteins located within influenza virus, in contrast to the glycoproteins present on the surface, show a high degree of conservation. Epitopes within these internal proteins often stimulate T-cell-mediated immune responses [57]. As a result, vaccines stimulating influenza specific T-cell immunity have been considered as candidates for a universal influenza vaccine [66].

SARS-CoV-1 infection survivors have been found to have a persistent CTL response to SARS-CoV-1 nucleocapsid two years after infection. [42] This suggests that the same approach could be applied to SARS-CoV-2 which has conserved regions in nucleocapsid which is located within the virus (see multiple sequence alignment in Supplementary Material Fig. 7 and Supplementary Material Fig. 8). Antigenic escape allows a virus to retain fitness despite an immune response to vaccination [20]. Picking conserved regions for vaccine targeting is an important part of mitigating this problem. Coronavirus spike protein, for example, may be particularly susceptible to mutation meaning that antigenic escape would be likely if the spike protein was targeted by a coronavirus vaccine, making it difficult to achieve durable protection. [74] A recent paper conducted a population genetic analysis of 103 SARS-CoV-2 genomes showing that the virus has evolved into two major types: L and S, with changes in their relative frequency after the outbreak possibly due to human intervention resulting in selection pressure [62].

We took all possible 424 9mer peptide sequences from the SARS-CoV-2 nucleocapsid protein sequences available and evaluated each peptide for HLA restriction using NetMHC 4.0 and NetMHCpan 4.0 [65][30][1]. We analyzed 9mer peptide sequences because these are often associated with superior MHC binding properties than Class I peptides of other lengths [63][18]. We found 53 unique peptides with predicted binding below 500nM from NetMHC 4.0 and/or NetMHCpan 4.0. These results are shown in Supplementary Material Table 6Supplementary Material Table 7Supplementary Material Table 8 and Supplementary Material Table 9.

We proceeded to determine the predicted HLA population coverage of a vaccine incorporating all 53 peptides using median values of the ANN, SMM, NetMHC 4.0 and NetMHCpan 4.0 algorithms hosted by IEDB [65]. These 53 peptides, taken together, had predicted HLA coverage of greater than 97% of the world’s population as shown in Supplementary Material Table 10. We also calculated HLA coverage based on alleles specific to populations in China and found that coverage across those individuals could be expected to be within 3% percent of the world wide coverage estimate as shown in Supplementary Material Table 11. This same population coverage could be achieved with 16 of the 53 unique peptides as shown in Table 4.

Table 4. This set of 16 unique peptides represents the minimum number required to achieve >95% world-wide population coverage. The starting position is within the nucleocapsid. Top binding affinity predictions chosen via NetMHC 4.0 or NetMHCpan 4.0. Peptide sequences colored in red have literature references as known in vitro binders to the predicted allele match (see text).

Sars cov 2 peptides

Seven of the 53 peptides with a predicted HLA match have been tested in vitro for HLA binding affinity by various researchers [65]. These binding affinity assays were originally performed with the SARS virus during a previous outbreak. Specific literature references for these in vitro assays for each peptide sequence are as follows: ASAFFGMSR, LSPRWYFYY, QQQGQTVTK: [53], FPRGQGVPI: [53][26][46][60], GMSRIGMEV: [26][64][13][41][12], KTFPPTEPK: [53][26][45][60][6] and LLLDRLNQL: [41][13][12][64][78]. These seven peptides are shown in red in Supplementary Material Table 6 and Supplementary Material Table 7.

The remaining 46 SARS-CoV-2 peptides listed in could also be further qualified as potential vaccine candidates by confirming MHC binding predictions by in vitro binding affinity and/or binding stability studies [54][52][26]. Another approach to evaluating the 53 SARS-CoV-2 candidate vaccine peptides though in vitro testing is also possible.

As we have shown in this paper, a peptide targeted by EBOV controllers could form the basis of a preventative vaccine for EBOV. ELISPOT analysis of PBMCs taken from the peripheral blood of COVID-19 controllers and progressors to assess the presence of a differential response to the 53 peptides could lead to a broadly applicable protective CTL vaccine against SARS-CoV-2 by incorporating peptides into the vaccine that are more commonly targeted for CD8+ attack by the controllers versus the progressors. A peptide vaccine for SARS-CoV-2, unlike a typical antibody vaccine, is not limited to virus surface antigen targets. This provides opportunities to attack other targets on SARS-CoV-2 besides spike which may be prone to mutation [74].

In addition, a peptide vaccine mitigates the risk of Antibody Disease Enhancement (ADE) seen in the context of a non-neutralizing antibody response to a whole protein vaccine [75][55]. Also, neutralizing antibodies directed against spike protein in SARS-CoV-1 patients have been associated with an increased risk of Acute Lung Injury (ALI)[35]. Specifically, patients succumbing to SARS-CoV-1 were found to develop a neutralizing antibody (NAb) response to spike protein faster than survivors after the onset of symptoms and the NAb titers were higher in the patients who died compared with those who recovered[76]. To the extent to which antibody vaccines producing an antibody response against the spike protein in SARS-CoV-2 could increase the risk of ALI, this risk could also be mitigated by a using peptide vaccine as an alternative approach.

The extent of the COVID-19 outbreak should allow many more controllers to be identified than the thirty individuals studied by Sakabe and the seven individuals identified in the Peng study [42][50]. Furthermore, Sakebe and Peng did not report progressor data perhaps because of the difficulty in obtaining blood samples from those patients. If researchers act now during the COVID-19 outbreak, perhaps controller and progressor blood samples could be collected and prospectively analyzed, quickly creating a database of optimal candidate Class I peptides for inclusion into a CTL vaccine with potentially broad HLA coverage for subsequent rapid manufacture and deployment. It would be interesting to see the extent to which the peptides favored by controllers appear on SARS-CoV-2 nucleocapsid, making SARS-CoV-2 a second example, across two different viruses, of controllers exhibiting CTL attack preferentially on the nucleocapsid protein.

Declaration of Competing Interest

CV Herst, Scott Burkholz, Lu Wang, Peter Lloyd and Reid Rubsamen are employees of Flow Pharma, Inc. all receiving cash and stock compensation. Alessandro Sette, Paul Harris, William Chao and Tom Hodge are members of Flow Pharma’s Scientific Advisory Board. Alessandro Sette has received cash and stock compensation as an SAB member. Richard Carback and Serban Ciotlos are consultants to Flow Pharma, both receiving cash and stock compensation. John Sidney works with Alessandro Sette at the La Jolla Institute of Allergy and Immunology. Flow Pharma, Inc. has previously contracted with the La Jolla Institute of Allergy and Immunology to support other research not related to this study funded under STTR contract CBD18-A-002-0016. Reid Rubsamen, CV Herst, Scott Burkholz, Lu Wang, Peter Lloyd, Richard Carback, Serban Ciotlos and Tom Hodge are named inventors on various issued and pending patents relating to Flow Pharma’s technology. All of the rights to all of these patents have been assigned by each of the inventors to Flow Pharma. Shane Massey, Trevor Brasel, Edecio Cunha-Neto and Daniela Rosa have nothing to declare.

Acknowlegements

All animal handling was done in accordance with NIH and institutional animal care and use guidelines by Aragen Bio-sciences in Morgan Hill California and the University of Texas, Medical Branch, Galveston Texas working in conjunction with the Galveston National Laboratory. The research was funded by Flow Pharma, Inc.

 
Galveston National Laboratory
The Galveston National Laboratory (GNL) is a sophisticated high containment research facility that serves as a critically important resource in the global fight against infectious diseases.

Flow Pharma today announced that researchers at the University of Texas Medical Branch at Galveston (UTMB) will begin testing Flow Pharma’s FlowVax-based COVID-19 treatment candidate by challenging nonhuman primates with SARS-CoV-2, the virus that causes COVID-19 in humans, after the animals are vaccinated this month with FLOVID-20. FlowVax biomedicines utilize Flow Pharma’s patented Size Exclusion Antigen Presentation Control (SEAPAC(TM)) technology based on the benefits of making vaccine microspheres the same size as human white blood cells.

“We are happy to be working with Flow Pharma on the pre-clinical testing phase of their FlowVax COVID-19 development program. Our team has extensive experience working with a variety of viruses in various pre-clinical models and is currently focusing on animal models for COVID-19. We are very fortunate at UTMB in that we have access to the Galveston National Laboratory that allow us to conduct studies like these safely and at a high level of quality to ensure data integrity for sponsors like Flow Pharma,” said Trevor Brasel, Ph.D., M(ASCP)CM, Assistant Professor, Department of Microbiology and Immunology, UTMB.

Dr. Brasel is author/co-author of over 70 peer-reviewed articles, editorials, book chapters, and poster/podium presentations on a broad range of topics including bioaerosol science and vaccine efficacy testing against select agents. To date, no vaccines or therapeutics have been approved for human use against SARS-CoV-2, the virus that causes COVID-19. Dr. Brasel’s ultimate goal is FDA approval of high-profile medical countermeasures specific for biodefense-related and emerging infectious diseases.

“We designed FlowVax COVID-19 to rely on killer T-cells rather than antibodies to fight virus infections,” said Charles Herst, Ph.D., Chief Science Officer, Flow Pharma “This allows us to more easily target the portions of the virus least likely to mutate, because mutations can allow a virus to hide from the antibody vaccine. We took the same approach to the design of FlowVax Ebola which successfully protected mice from Ebolavirus infection in studies also performed at UTMB under Dr. Brasel’s team. The nonhuman primates at UTMB will be challenged with the same virus that causes COVID-19 in humans providing a realistic test of FlowVax COVID-19 before we begin testing in human subjects.”

“We have successfully completed many pre-clinical studies with the team at UTMB” said Reid Rubsamen, M.D., CEO, Flow Pharma. “We are very fortunate to have access to one of the first animal models in the world being developed to test vaccines designed to prevent COVID-19.”

About the University of Texas Medical Branch and the Galveston National Laboratory

The Galveston National Laboratory (GNL) is a sophisticated high containment research facility that serves as a critically important resource in the global fight against infectious diseases. The GNL is located on the campus of the University of Texas Medical Branch and operates under the umbrella of UTMB’s Institute for Human Infections and Immunity. The GNL is an anchor lab of the NIAID Biodefense Laboratory Network and is one of only two National Laboratories with Biosafety Level 4 (BSL-4) capabilities located on a U.S. university campus.

About Flow Pharma

Flow Pharma, Inc. is a San Francisco Bay Area based, biotechnology company using artificial intelligence to guide selection of neoantigen peptide targets on cancer cells or virus-infected cells for attack by the patient’s own, native immune system. These peptides can then be loaded into the FlowVax(TM) platform for administration by injection.

Flow Pharma, Inc. is conducting gene sequencing studies with neoantigen identification on breast cancer patients in the U.S. and cervical cancer patients in China, in preparation for conducting Phase I/II clinical trials planned for later this year. Neoantigens are small peptide markers expressed on cancer cells as a result of a cancer-causing viral infection or mutation of the DNA in normal cells, agents that cause transformation into cancer cells.

Forward Looking Statements

This press release may contain forward-looking statements, including information about management’s view of Flow Pharma, Inc. (“the Company”), future expectations, plans and prospects. In particular, when used in the preceding discussion, the words “believes,” “expects,” “intends,” “plans,” “anticipates,” or “may,” and similar conditional expressions are intended to identify forward-looking statements. Any statements made in this presentation other than those of historical fact, about an action, event or development, are forward-looking statements. These statements involve known and unknown risks, uncertainties and other factors, which may cause the results of the Company, its subsidiaries and concepts to be materially different than those expressed or implied in such statements. Unknown or unpredictable factors also could have material adverse effects on the Company’s future results. The forward-looking statements included in this presentation are made only as of the date hereof. The Company cannot guarantee future results, levels of activity, performance or achievements. Accordingly, you should not place undue reliance on these forward-looking statements. Finally, the Company undertakes no obligation to update these statements after the date of this release, except as required by law, and also takes no obligation to update or correct information prepared by third parties that are not paid for by Flow Pharma, Inc.